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| ANGIOTENSIN-I-CONVERTING ENZYME IN HUMAN CIRCULATING MONONUCLEAR-CELLS - GENETIC-POLYMORPHISM OF EXPRESSION IN LYMPHOCYTES-T |
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| Author(s): COSTEROUSSE O, ALLEGRINI J, LOPEZ M, ALHENCGELAS F |
| Source: BIOCHEMICAL JOURNAL Volume: 290 Pages: 33-40 Part: Part 1 Published: FEB 15 1993 |
| Times Cited: 376 References: 55 |
| Abstract: The expression of angiotensin-I converting enzyme (ACE; EC 3.4.15.1) in human circulating mononuclear cells was studied. T-lymphocytes contained the highest level of enzyme, approx. 28 times more per cell than monocytes. No activity was detected in B-lymphocytes. ACE was present mainly in the microsomal fraction, where it was found to be the major membrane-bound bradykinin-inactivating enzyme. An mRNA for ACE was detected and characterized after reverse transcription and amplification by PCR in T-lymphocytes and several T-cell leukaemia cell lines. We have previously observed that the interindividual variability in the levels of ACE in plasma is, in part, genetically determined and influenced by an insertion/deletion polymorphism of the ACE gene. To investigate the mechanisms involved in the regulation of ACE biosynthesis, the ACE levels of T-lymphocytes from 35 healthy subjects having different ACE genotypes were studied. These levels varied widely between individuals but were highly reproducible and influenced by the polymorphism of the ACE gene. T-lymphocyte levels of ACE were significantly higher in subjects who were homozygote for the deletion than in the other subjects. These results show that ACE is expressed in T-lymphocytes and indicate that the level of ACE expression in cells synthesizing the enzyme is genetically determined. |
| Document Type: Article |
| Language: English |
Addresses:
1. INSERM, U367, 17 RUE FER A MOULIN, F-75005 PARIS, FRANCE
2. INSERM, U76, F-75005 PARIS, FRANCE |
| Publisher: PORTLAND PRESS, 59 PORTLAND PLACE, LONDON, ENGLAND W1N 3AJ |
| Subject Category: Biochemistry & Molecular Biology |
| IDS Number: KN694 |
| ISSN: 0264-6021 |
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