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| GERM-LINE TRANSMISSION AND EXPRESSION OF A HUMAN-DERIVED YEAST ARTIFICIAL CHROMOSOME |
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| Author(s): JAKOBOVITS A, MOORE AL, GREEN LL, VERGARA GJ, MAYNARDCURRIE CE, AUSTIN HA, KLAPHOLZ S |
| Source: NATURE Volume: 362 Issue: 6417 Pages: 255-258 Published: MAR 18 1993 |
| Times Cited: 102 References: 22 |
| Abstract: INTRODUCTION of DNA fragments, hundreds of kilobases in size, into mouse embryonic stem (ES) cells would greatly advance the ability to manipulate the mouse genome. Mice generated from such modified cells would permit investigation of the function and expression of very large or crudely mapped genes. Large DNA molecules cloned into yeast artificial chromosomes (YACs) are stable and genetically manipulable within yeast1, suggesting yeast-cell fusion as an ideal method for transferring large DNA segments into mammalian cells. Introduction of YACs into different cell types by this technique has been reported2-8; however, the incorporation of yeast DNA along with the YAC has raised doubts as to whether ES cells, modified in this way. would be able to recolonize the mouse germ line5. Here we provide, to our knowledge, the first demonstration of germ-line transmission and expression of a large human DNA fragment, introduced into ES cells by fusion with yeast spheroplasts. Proper development was not impaired by the cointegration of a large portion of the yeast genome with the YAC. |
| Document Type: Article |
| Language: English |
| Reprint Address: JAKOBOVITS, A (reprint author), CELL GENESYS INC, 322 LAKESIDE DR, FOSTER CITY, CA 94404 USA |
| Publisher: MACMILLAN MAGAZINES LTD, PORTERS SOUTH, 4 CRINAN ST, LONDON, ENGLAND N1 9XW |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: KT026 |
| ISSN: 0028-0836 |
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