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| INDUCTION OF THE METASTATIC PHENOTYPE BY TRANSFECTION OF A BENIGN RAT MAMMARY EPITHELIAL-CELL LINE WITH THE GENE FOR P9KA, A RAT CALCIUM-BINDING PROTEIN, BUT NOT WITH THE ONCOGENE EJ-RAS-1 |
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| Author(s): DAVIES BR, DAVIES MPA, GIBBS FEM, BARRACLOUGH R, RUDLAND PS |
| Source: ONCOGENE Volume: 8 Issue: 4 Pages: 999-1008 Published: APR 1993 |
| Times Cited: 167 References: 49 |
| Abstract: The rat mammary epithelial cell tine, Rama 37, yields benign, non-metastasizing adenomatous tumours in syngeneic Wistar-Furth rats. Transfection of this line with a drug resistance plasmid containing both the gene for resistance to Geneticin (neo) and the gene for p9Ka (pSV2neo-p9Ka), a rat calcium-binding protein, or with a similar plasmid containing neo and the oncogene EJ-ras-1 (pSV2neo-ras) yields drug-resistant transformants that express high levels of the p9Ka or EJ-ras-I mRNAs and proteins. These transfected cells all produce tumours when injected at subcutaneous sites with a shorter median latent period than the tumours produced by the parental untransfected Rama 37 cells in syngeneic hosts. Cells transfected with pSV2neo-p9Ka yield a higher incidence of tumours than untransfected Rama 37 cells, many of which metastasize to lungs and/or lymph nodes in syngeneic rats. However, cells transfected with pSV2neo-ras or pSV2neo plasmid alone yield tumours that fail to metastasize. Immunofluorescent studies suggest an association of p9Ka with the cytoskeleton, as depicted by F-actin staining with the reagent phalloidin. It is suggested that the transfection of copies of the gene for the rat calcium-binding protein p9Ka can enhance the tumorigenic potential and induce the metastatic phenotype in this rat mammary model, whereas transfection of control plasmid DNA or the oncogene EJ-ras-I fails to induce the metastatic phenotype, although EJ-ras-I transfectants, like those containing p9Ka, possess increased growth properties in vivo. |
| Document Type: Article |
| Language: English |
Addresses:
1. UNIV LIVERPOOL, DEPT BIOCHEM, CANCER & POLIO RES FUND LABS, LIVERPOOL L69 3BX, ENGLAND |
| Publisher: STOCKTON PRESS, HOUNDMILLS, BASINGSTOKE, HAMPSHIRE, ENGLAND RG21 6XS |
| Subject Category: Biochemistry & Molecular Biology; Oncology; Cell Biology; Genetics & Heredity |
| IDS Number: KT220 |
| ISSN: 0950-9232 |
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