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| HUMAN RECOMBINANT INTERLEUKIN-1-BETA UP-REGULATES ELASTIN GENE-EXPRESSION IN DERMAL FIBROBLASTS - EVIDENCE FOR TRANSCRIPTIONAL REGULATION INVITRO AND INVIVO |
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| Author(s): MAUVIEL A, CHEN YQ, KAHARI VM, LEDO I, WU M, RUDNICKA L, UITTO J |
| Source: JOURNAL OF BIOLOGICAL CHEMISTRY Volume: 268 Issue: 9 Pages: 6520-6524 Published: MAR 25 1993 |
| Times Cited: 65 References: 34 |
| Abstract: The effects of human recombinant interleukin (IL)-1beta on elastin gene expression were studied in human skin fibroblast cultures by Northern hybridization and transient transfection experiments. Incubation of the cells with IL-1beta elevated the elastin mRNA stead-state levels by approximately 3- to 4-fold. A similar increase was noted at the protein level, when estimated by indirect immunofluorescence of cultured cells. This effect was independent of the on-going protein synthesis, as tested by incubation with cycloheximide. Transient transfections of the dermal fibroblasts with a human elastin promoter/chloramphenicol acetyltransferase (CAT) reporter gene construct suggested transcriptional regulation, since the CAT activity in cells incubated with IL-1beta was similarly increased approximately 3-fold. Enhancement of the human elastin promoter activity by IL-1beta was also noted in fibroblast cultures established from the skin and lungs of transgenic mice which have integrated the human promoter/CAT construct into their genome and express it in a tissue-specific manner. Furthermore, subcutaneous injection of IL-1beta to the mice resulted in a approximately 4-fold elevation of the CAT activity in the skin after a 30-h incubation, as compared to the CAT activity in the skin of control animals. Collectively, these data indicate that IL-1beta up-regulates elastin gene expression in fibroblast cultures as well as in the skin in vivo, and the activation occurs at the transcriptional level. |
| Document Type: Article |
| Language: English |
Addresses:
1. THOMAS JEFFERSON UNIV, JEFFERSON MED COLL, DEPT DERMATOL, 233 S 10TH ST, RM 450 BLSB, PHILADELPHIA, PA 19107 USA
2. THOMAS JEFFERSON UNIV, JEFFERSON INST MOLEC MED, MOLEC DERMATOL SECT, PHILADELPHIA, PA 19107 USA |
| Publisher: AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 |
| Subject Category: Biochemistry & Molecular Biology |
| IDS Number: KT368 |
| ISSN: 0021-9258 |
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