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| HIGH-LEVELS OF HIV-1 IN PLASMA DURING ALL STAGES OF INFECTION DETERMINED BY COMPETITIVE PCR |
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| Author(s): PIATAK M, SAAG MS, YANG LC, CLARK SJ, KAPPES JC, LUK KC, HAHN BH, SHAW GM, LIFSON JD |
| Source: SCIENCE Volume: 259 Issue: 5102 Pages: 1749-1754 Published: MAR 19 1993 |
| Times Cited: 997 References: 71 |
| Abstract: Quantitative competitive polymerase chain reaction (QC-PCR) methods were used to quantify virion-associated human immunodeficiency virus type-1 (HIV-1) RNA in plasma from 66 patients with Centers for Disease Control stage I to IVC1 infection. HIV-1 RNA, ranging from 100 to nearly 22,000,000 copies per milliliter of plasma (corresponding to 50 to 11,000,000 virions per milliliter), was readily quantified in all subjects, was significantly associated with disease stage and CD4+ T cell counts, and decreased by as much as 235-fold with resolution of primary infection or institution of antiretroviral therapy. Plasma virus levels determined by QC-PCR correlated with, but exceeded by an average of 60,000-fold, virus titers measured by endpoint dilution culture. Quantitation of HIV-1 in plasma by QC-PCR may be useful in assessing the efficacy of antiretroviral agents, especially in early stage disease when conventional viral markers are often negative. |
| Document Type: Article |
| Language: English |
Addresses:
1. GENELABS TECHNOL INC, DIV HIV & EXPLORATORY RES, REDWOOD CITY, CA 94063 USA
2. UNIV ALABAMA, DEPT MED, DIV HEMATOL ONCOL, BIRMINGHAM, AL 35294 USA
3. UNIV ALABAMA, DEPT MED, DIV INFECT DIS, BIRMINGHAM, AL 35294 USA |
| Publisher: AMER ASSOC ADVANCEMENT SCIENCE, 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: KT810 |
| ISSN: 0036-8075 |
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