| | |  | | | | Record from Web of Science® | | | | | | |
| GERM LINE TRANSMISSION OF A YEAST ARTIFICIAL CHROMOSOME SPANNING THE MURINE ALPHA(1)(I) COLLAGEN LOCUS |
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| Author(s): STRAUSS WM, DAUSMAN J, BEARD C, JOHNSON C, LAWRENCE JB, JAENISCH R |
| Source: SCIENCE Volume: 259 Issue: 5103 Pages: 1904-1907 Published: MAR 26 1993 |
| Times Cited: 102 References: 35 |
| Abstract: Molecular complementation of mutant phenotypes by transgenic technology is a potentially important tool for gene identification. A technology was developed that allows the transfer of a physically intact yeast artificial chromosome (YAC) into the germ line of the mouse. A purified 150-kilobase YAC encompassing the murine gene Col1a1 was efficiently introduced into embryonic stem (ES) cells via lipofection. Chimeric founder mice were derived from two transfected ES cell clones. These chimeras transmitted the full length transgene through the germ line, generating two transgenic mouse strains. Transgene expression was visualized as nascent transcripts in interphase nuclei and quantitated by ribonuclease protection analysis. Both assays indicated that the transgene was expressed at levels comparable to the endogenous collagen gene. |
| Document Type: Article |
| Language: English |
| Reprint Address: STRAUSS, WM (reprint author), MIT, WHITEHEAD INST BIOMED RES, 9 CAMBRIDGE CTR, CAMBRIDGE, MA 02142 USA |
Addresses:
1. MIT, DEPT BIOL, CAMBRIDGE, MA 02142 USA
2. UNIV MASSACHUSETTS, SCH MED, WORCESTER, MA 01655 USA |
| Publisher: AMER ASSOC ADVANCEMENT SCIENCE, 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: KU172 |
| ISSN: 0036-8075 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |