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| A DESIGNED METAL-BINDING PROTEIN WITH A NOVEL FOLD |
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| Author(s): PESSI A, BIANCHI E, CRAMERI A, VENTURINI S, TRAMONTANO A, SOLLAZZO M |
| Source: NATURE Volume: 362 Issue: 6418 Pages: 367-369 Published: MAR 25 1993 |
| Times Cited: 162 References: 24 |
| Abstract: A MAJOR challenge in protein design is to create stable scaffolds into which tailored functions can be introduced. Here we present the design, synthesis and characterization of a 61-residue all-beta protein: the minibody. We used a portion of the heavy chain variable domain of an immunoglobulin as a template, obtaining a molecule with a novel beta-sheet scaffold and two regions corresponding to the hypervariable loops H1 and H2. To exploit the potential for creating functional centres in the minibody, we engineered a metal-binding site into it. This site is formed by one histidine in H1 and two in H2. The protein is folded, compact and able to bind metal, thus representing the first designed beta-protein with a novel fold and a tailored function. By randomizing the sequence of the hypervariable loops, we are using the minibody scaffold to construct a conformationally constrained peptide library displayed on phage. |
| Document Type: Article |
| Language: English |
Addresses:
1. IST RIC BIOL MOLEC P ANGELETTI, DEPT GENET, VIA PONTINA KM 306, I-00040 POMEZIA, ITALY
2. IST RIC BIOL MOLEC P ANGELETTI, DEPT BIOCHEM, I-00040 POMEZIA, ITALY
3. IST RIC BIOL MOLEC P ANGELETTI, DEPT BIOCOMP, I-00040 POMEZIA, ITALY |
| Publisher: MACMILLAN MAGAZINES LTD, PORTERS SOUTH, 4 CRINAN ST, LONDON, ENGLAND N1 9XW |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: KU176 |
| ISSN: 0028-0836 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |