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| GLOBAL REGULATION OF GENE-EXPRESSION IN ESCHERICHIA-COLI |
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| Author(s): CHUANG SE, DANIELS DL, BLATTNER FR |
| Source: JOURNAL OF BACTERIOLOGY Volume: 175 Issue: 7 Pages: 2026-2036 Published: APR 1993 |
| Times Cited: 141 References: 58 |
| Abstract: Global transcription responses of Escherichia coli to various stimuli or genetic defects were studied by measuring mRNA levels in about 400 segments of the genome. Measuring mRNA levels was done by analyzing hybridization to DNA dot blots made with overlapping lambda clones spanning the genome of E. coli K-12. Conditions examined included isopropyl-beta-D-thiogalactopyranoside (IPTG) induction, heat shock, osmotic shock, starvation for various nutrients, entrance of cells into the stationary phase of growth, anaerobic growth in a tube, growth in the gnotobiotic mouse gut, and effects of pleiotropic mutations rpoH, himA, topA, and crp. Most mapped genes known to be regulated by a particular situation were successfully detected. In addition, many chromosomal regions containing no previously known regulated genes were discovered that responded to various stimuli. This new method for studying globally regulated genetic systems in E. coli combines detection, cloning, and physical mapping of a battery of coregulated genes in one step. |
| Document Type: Article |
| Language: English |
Addresses:
1. UNIV WISCONSIN, GENET LAB, 445 HENRY MALL, MADISON, WI 53706 USA |
| Publisher: AMER SOC MICROBIOLOGY, 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 |
| Subject Category: Microbiology |
| IDS Number: KU490 |
| ISSN: 0021-9193 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |