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| MATERNAL-SPECIFIC METHYLATION OF THE IMPRINTED MOUSE IGF2R LOCUS IDENTIFIES THE EXPRESSED LOCUS AS CARRYING THE IMPRINTING SIGNAL |
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| Author(s): STOGER R, KUBICKA P, LIU CG, KAFRI T, RAZIN A, CEDAR H, BARLOW DP |
| Source: CELL Volume: 73 Issue: 1 Pages: 61-71 Published: APR 9 1993 |
| Times Cited: 405 References: 39 |
| Abstract: The mouse insulin-like growth factor type 2 receptor (Igf2r) is imprinted and expressed exclusively from the maternally inherited chromosome. To investigate whether methylation could function as the imprinting signal, we have cloned 130 kb from the Igf2r locus and searched for sequences methylated in a parental-specific manner. Two regions have been identified: region 1 contains the start of transcription and is methylated only on the silent paternal chromosome; region 2 is contained in an intron and is methylated only on the expressed maternal chromosome. Methylation of region 1 is acquired after fertilization, in contrast with the methylation of region 2, which is inherited from the female gamete. Methylation of region 2 may mark the maternal Igf2r locus in a manner that could act as an imprinting signal. These data suggest that the expressed locus carries a potential imprinting signal and imply that methylation is necessary for expression of the Igf2r gene. |
| Document Type: Article |
| Language: English |
| Reprint Address: STOGER, R (reprint author), RES INST MOLEC PATHOL, DR BOHR GASSE 7, A-1030 VIENNA, AUSTRIA |
Addresses:
1. HEBREW UNIV JERUSALEM, HADASSAH MED SCH, IL-91010 JERUSALEM, ISRAEL |
| Publisher: CELL PRESS, 1050 MASSACHUSETTES AVE, CIRCULATION DEPT, CAMBRIDGE, MA 02138 |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology |
| IDS Number: KW753 |
| ISSN: 0092-8674 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |