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| INHIBITION OF NEUROTRANSMITTER RELEASE BY C2-DOMAIN PEPTIDES IMPLICATES SYNAPTOTAGMIN IN EXOCYTOSIS |
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| Author(s): BOMMERT K, CHARLTON MP, DEBELLO WM, CHIN GJ, BETZ H, AUGUSTINE GJ |
| Source: NATURE Volume: 363 Issue: 6425 Pages: 163-165 Published: MAY 13 1993 |
| Times Cited: 260 References: 31 |
| Abstract: NEUROTRANSMITTER release is triggered by Ca2+ ions binding to an unknown Ca2+ receptor within presynaptic terminals1,2. Synaptotagmin, a Ca2+-binding protein of synaptic and other secretory vesicles3, has been proposed to mediate vesicle-plasma membrane interactions during neurotransmitter release4-9. Here we test this hypothesis using the giant synapse of the squid Loligo pealei, which because of its unusually large size and well established physiology is uniquely suited for dissecting presynaptic events10. We find that injection of peptides from the C2 domains of synaptotagmin into squid giant presynaptic terminals rapidly and reversibly inhibits neurotransmitter release. Our data are consistent with these peptides competitively blocking release after synaptic vesicle docking and indicate that Ca2+ probably initiates neurotransmitter release by regulating the interaction of synaptotagmin with an acceptor protein. |
| Document Type: Article |
| Language: English |
| Reprint Address: BOMMERT, K (reprint author), MARINE BIOL LAB, WOODS HOLE, MA 02543 USA |
Addresses:
1. MAX PLANCK INST BRAIN RES, DEPT NEUROCHEM, W-6000 FRANKFURT 71, GERMANY
2. UNIV TORONTO, DEPT PHYSIOL, TORONTO M5S 1A8, ONTARIO CANADA
3. DUKE UNIV, MED CTR, DEPT NEUROBIOL, DURHAM, NC 27710 USA
4. NIH, DEV NEUROBIOL LAB, BETHESDA, MD 20892 USA |
| Publisher: MACMILLAN MAGAZINES LTD, PORTERS SOUTH, 4 CRINAN ST, LONDON, ENGLAND N1 9XW |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: LB801 |
| ISSN: 0028-0836 |
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