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| PROPHYLACTIC EFFECTS OF RECOMBINANT HUMAN SUPEROXIDE-DISMUTASE IN NEONATAL LUNG INJURY |
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| Author(s): DAVIS JM, ROSENFELD WN, SANDERS RJ, GONENNE A |
| Source: JOURNAL OF APPLIED PHYSIOLOGY Volume: 74 Issue: 5 Pages: 2234-2241 Published: MAY 1993 |
| Times Cited: 63 References: 33 |
| Abstract: To determine if recombinant human Cu-Zn superoxide dismutase (rhSOD) would prevent acute lung injury caused by hyperoxia and barotrauma, 26 newborn piglets were studied. Ten piglets were hyperventilated (arterial PCO2 15-20 Torr) with 100% O2 for 48 h. A second group received identical treatment for 4 h (n = 2) or 48 h (n = 8) but was given 5 mg/kg of rhSOD intratracheally at time 0. Six piglets were normally ventilated (arterial PCO2 40-45 Torr) for 48 h with 21% O2. Pulmonary function and tracheal aspirates were examined at time 0 and at 24 and 48 h, and bronchoalveolar lavage was performed at 48 h. In piglets treated with hyperoxia and hyperventilation, lung compliance decreased 42%, and tracheal aspirates showed an increase in neutrophil chemotactic activity (32%), total cell counts (135%), elastase activity (93%), and albumin concentration (339%) over 48 h (P < 0.05). All variables were significantly lower in rhSOD-treated piglets and comparable to normoxic control values. Surfactant remained active in all groups. Immunohistochemistry demonstrated that at 48 h significant rhSOD was distributed homogeneously in terminal airways. Adding rhSOD to tracheal aspirates of hyperoxic hyperventilated piglets did not alter neutrophil chemotaxis, suggesting that rhSOD protected the lung by reducing the production of chemotactic mediators. Results indicate that acute lung injury caused by 48 h of hyperoxia and hyperventilation is significantly ameliorated by prophylactic intratracheal administration of rhSOD. |
| Document Type: Article |
| Language: English |
Addresses:
1. SUNY, WINTHROP UNIV HOSP, SCH MED, DEPT PEDIAT NEONATOL, MINEOLA, NY 11501 USA
2. SUNY, WINTHROP UNIV HOSP, SCH MED, PULM RES INST, MINEOLA, NY 11501 USA
3. UNIV ROCHESTER, SCH MED, DEPT PEDIAT, ROCHESTER, NY 14642 USA
4. UNIV ROCHESTER, SCH MED, STRONG CHILDRENS RES CTR, ROCHESTER, NY 14642 USA
5. BIOTECHNOL GEN CORP, NEW YORK, NY 10001 USA |
| Publisher: AMER PHYSIOLOGICAL SOC, 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 |
| Subject Category: Physiology; Sport Sciences |
| IDS Number: LD699 |
| ISSN: 8750-7587 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |