| | |  | | | | Record from Web of Science® | | | | | | |
| ANTISENSE GENE INHIBITION BY OLIGONUCLEOTIDES CONTAINING C-5 PROPYNE PYRIMIDINES |
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| Author(s): WAGNER RW, MATTEUCCI MD, LEWIS JG, GUTIERREZ AJ, MOULDS C, FROEHLER BC |
| Source: SCIENCE Volume: 260 Issue: 5113 Pages: 1510-1513 Published: JUN 4 1993 |
| Times Cited: 353 References: 17 |
| Abstract: Phosphorothioate oligodeoxynucleotides containing the C-5 propyne analogs of uridine and cytidine bind RNA with high affinity and are potent antisense inhibitors of gene expression. In a cellular assay, gene-specific antisense inhibition occurred at nanomolar concentrations of oligonucleotide, was dose-dependent and exquisitely sensitive to sequence mismatches, and was correlated with the melting temperature and length of oligonucleotide. Activity was independent of RNA target site and cell type but was detectable only when the oligonucleotides were microinjected or delivered with cell-permeabilizing agents. These oligonucleotides may have important applications in therapy and in studies of gene function. |
| Document Type: Article |
| Language: English |
| Reprint Address: WAGNER, RW (reprint author), GILEAD SCI INC, 353 LAKESIDE DR, FOSTER CITY, CA 94404 USA |
| Publisher: AMER ASSOC ADVANCEMENT SCIENCE, 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: LF058 |
| ISSN: 0036-8075 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |