| P53-CATALYZED ANNEALING OF COMPLEMENTARY SINGLE-STRANDED NUCLEIC-ACIDS |
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| Author(s): OBEROSLER P, HLOCH P, RAMSPERGER U, STAHL H |
| Source: EMBO JOURNAL Volume: 12 Issue: 6 Pages: 2389-2396 Published: JUN 1993 |
| Times Cited: 230 References: 66 |
| Abstract: p53 has been reported to inhibit the DNA helicase intrinsic to simian virus 40 large tumor antigen (T antigen). We found that inhibition is not restricted to T antigen, but also affects several other DNA and RNA helicases. Complexing of the helicases by the p53 protein as a possible inactivation mechanism could be excluded. Instead, the anti-helicase activity can be explained by our finding that p53 binds with high affinity to single-stranded nucleic acids and has a strong DNA.DNA and RNA.RNA annealing activity. We could also show that p53 is able to alter the secondary structure of RNA and/or to influence dynamic RNA - RNA interactions. These results, and the fact that the affinity of p53 to RNA is about one order of magnitude higher than to single-stranded DNA, imply an RNA-specific function of p53 in vivo. |
| Document Type: Article |
| Language: English |
Addresses:
1. UNIV CONSTANCE, FAK BIOL, W-7750 CONSTANCE, GERMANY |
| Publisher: OXFORD UNIV PRESS UNITED KINGDOM, WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology |
| IDS Number: LF938 |
| ISSN: 0261-4189 |