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| DETERMINATION OF THE GROWTH FRACTION IN NON-HODGKINS-LYMPHOMAS BY MONOCLONAL-ANTIBODY KI-S5 DIRECTED AGAINST A FORMALIN-RESISTANT EPITOPE OF THE KI-67 ANTIGEN |
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| Author(s): KREIPE H, WACKER HH, HEIDEBRECHT HJ, HAAS K, HAUBERG M, TIEMANN M, PARWARESCH R |
| Source: AMERICAN JOURNAL OF PATHOLOGY Volume: 142 Issue: 6 Pages: 1689-1694 Published: JUN 1993 |
| Times Cited: 75 References: 23 |
| Abstract: Relevant prognostic information can be added to the histological classification of non-Hodgkin's lymphoma (NHL) by the determination of the growth fraction In unfixed fresh tissue samples the Ki-67 antigen has proven its utility as an operational marker of proliferative activity in NHL. We have generated a monoclonal antibody directed against a formalin-resistant epitope of the Ki-67 antigen, designated Ki-S5. The immunoreactivity of Ki-S5 is confined to the nuclei of proliferating cells and, unlike Ki-67, no cross-reactivity with cytoplasmic antigens of epithelial cells occurs. In 100 cases of different NHL types, parallel staining of Ki-67 and Ki-S5 antigen yielded almost identical results in fresh tissues and fixed tissues, respectively (P < 0,00001; r = 0. 95). In some cases, the Ki-S5 labeling index exceeded that of Ki-67, most probably due to a partial degradation of the epitope recognized by Ki-67 in frozen stored tissue samples. Significant differences in the median Ki-S5 labeling index could be demonstrated between high grade (>50%) and low grade malignant NHL (less-than-or-equal-to 30%), although the proliferative activity varied to a considerable range within identical histological categories (P < 0.001). We conclude that Ki-S5 provides a reliable means to assess proliferation in Paraffin-embedded NHL specimens. Retrospecclinical outcome are thus rendered possible using archival routinely processed tissue samples. |
| Document Type: Article |
| Language: English |
| Reprint Address: KREIPE, H (reprint author), CHRISTIAN ALBRECHTS UNIV KIEL, INST PATHOL, MICHAELISSTR 11, W-2300 KIEL 1, GERMANY |
Addresses:
1. CHRISTIAN ALBRECHTS UNIV KIEL, INST HEMATOPATHOL, W-2300 KIEL 1, GERMANY |
| Publisher: AMER SOC INVESTIGATIVE PATHOLOGY, INC, 428 EAST PRESTON ST, BALTIMORE, MD 21202-3993 |
| Subject Category: Pathology |
| IDS Number: LF978 |
| ISSN: 0002-9440 |
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