| | |  | | | | Record from Web of Science® | | | | | | |
| DNA-SEQUENCE DETERMINATION BY HYBRIDIZATION - A STRATEGY FOR EFFICIENT LARGE-SCALE SEQUENCING |
|
|
| Author(s): DRMANAC R, DRMANAC S, STREZOSKA Z, PAUNESKU T, LABAT I, ZEREMSKI M, SNODDY J, FUNKHOUSER WK, KOOP B, HOOD L, CRKVENJAKOV R |
| Source: SCIENCE Volume: 260 Issue: 5114 Pages: 1649-1653 Published: JUN 11 1993 |
| Times Cited: 135 References: 31 |
| Abstract: The concept of sequencing by hybridization (SBH) makes use of an array of all possible n-nucleotide oligomers (n-mers) to identify n-mers present in an unknown DNA sequence. Computational approaches can then be used to assemble the complete sequence. As a validation of this concept, the sequences of three DNA fragments, 343 base pairs in length, were determined with octamer oligonucleotides. Possible applications of SBH include physical mapping (ordering) of overlapping DNA clones, sequence checking, DNA fingerprinting comparisons of normal and disease-causing genes, and the identification of DNA fragments with particular sequence motifs in complementary DNA and genomic libraries. The SBH techniques may accelerate the mapping and sequencing phases of the human genome project. |
| Document Type: Article |
| Language: English |
Addresses:
1. ARGONNE NATL LAB, DIV BIOL & MED RES, ARGONNE, IL 60439 USA
2. CALTECH, DEPT BIOL, PASADENA, CA 91125 USA
3. INST MOLEC GENET & GENET ENGN, YU-11000 BELGRADE, YUGOSLAVIA |
| Publisher: AMER ASSOC ADVANCEMENT SCIENCE, 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: LG176 |
| ISSN: 0036-8075 |
|
| | | | | | | | | Record from Web of Science® | | | | | | | | | |