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| GROWTH-RATE PARADOX OF SALMONELLA-TYPHIMURIUM WITHIN HOST MACROPHAGES |
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| Author(s): ABSHIRE KZ, NEIDHARDT FC |
| Source: JOURNAL OF BACTERIOLOGY Volume: 175 Issue: 12 Pages: 3744-3748 Published: JUN 1993 |
| Times Cited: 70 References: 13 |
| Abstract: The growth rate of Salmonella typhimurium U937 within host macrophages was estimated by two independent methods. The extent to which ribosomal protein L12 is acetylated to produce ribosomal protein L7 changes markedly with the growth rate. By this measure, the intracellular bacteria appeared to be growing rapidly. Measurements of viable bacteria, however, indicated that the bacteria were growing slowly. A solution of this apparent growth rate paradox was sought by treating U937 cells infected with S. typhimurium X3306 with ampicillin or chloramphenicol to help determine the number of bacteria that were actively growing and dividing in the intracellular condition. Use of these antibiotics showed that by 2 h after invasion, the intracellular bacteria consisted of at least two populations, one static and the other rapidly dividing. This finding implies that previously described changes in the gene expression of S. typhimurium are important for the survival and/or multiplication of the bacteria within the macrophage. |
| Document Type: Article |
| Language: English |
Addresses:
1. UNIV MICHIGAN, SCH MED, DEPT MICROBIOL & IMMUNOL, ANN ARBOR, MI 48109 USA |
| Publisher: AMER SOC MICROBIOLOGY, 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 |
| Subject Category: Microbiology |
| IDS Number: LG985 |
| ISSN: 0021-9193 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |