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| THE DISTRIBUTION OF GLUTAMATE RECEPTORS IN CULTURED RAT HIPPOCAMPAL-NEURONS - POSTSYNAPTIC CLUSTERING OF AMPA-SELECTIVE SUBUNITS |
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| Author(s): CRAIG AM, BLACKSTONE CD, HUGANIR RL, BANKER G |
| Source: NEURON Volume: 10 Issue: 6 Pages: 1055-1068 Published: JUN 1993 |
| Times Cited: 264 References: 59 |
| Abstract: The distribution of several glutamate receptor subunits was investigated in cultured rat hippocampal neurons by in situ hybridization and immunocytochemistry. The AMPA/kainate-selective receptors GluR1-6 exhibited two patterns of mRNA expression: most neurons expressed GluR1, R2, and R6, whereas only about 20% expressed significant levels of GluR3, R4, and R5. By immunocytochemistry, the metabotropic glutamate receptor mGluR1alpha was detectable only in a subpopulation of GABAergic interneurons. GluR1 and GluR2/3 segregated to the somatodendritic domain within the first week in culture, even in the absence of synaptogenesis. Glutamate receptor-enriched spines developed later and were present only on presumptive pyramidal cells, not on GABAergic interneurons. Clusters of GluR1 and GluR2/3 completely colocalized and were restricted to a subset of postsynaptic sites. Thus, glutamate receptor subunits exhibit both a cell type-specific expression and a selective subcellular localization. |
| Document Type: Article |
| Language: English |
| Reprint Address: CRAIG, AM (reprint author), UNIV VIRGINIA, SCH MED, DEPT NEUROSCI, CHARLOTTESVILLE, VA 22908 USA |
Addresses:
1. JOHNS HOPKINS UNIV, SCH MED, HOWARD HUGHES MED INST, BALTIMORE, MD 21205 USA
2. JOHNS HOPKINS UNIV, SCH MED, DEPT NEUROSCI, BALTIMORE, MD 21205 USA |
| Publisher: CELL PRESS, 1050 MASSACHUSETTES AVE, CIRCULATION DEPT, CAMBRIDGE, MA 02138 |
| Subject Category: Neurosciences |
| IDS Number: LK373 |
| ISSN: 0896-6273 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |