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| AGROBACTERIUM-MEDIATED PRODUCTION OF TRANSGENIC RICE PLANTS EXPRESSING A CHIMERIC ALPHA-AMYLASE PROMOTER BETA-GLUCURONIDASE GENE |
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| Author(s): CHAN MT, CHANG HH, HO SL, TONG WF, YU SM |
| Source: PLANT MOLECULAR BIOLOGY Volume: 22 Issue: 3 Pages: 491-506 Published: JUN 1993 |
| Times Cited: 103 References: 33 |
| Abstract: We have successfully transferred and expressed a reporter gene driven by an alpha-amylase promoter in a japonica type of rice (Oryza sativa L. cv. Tainung 62) using the Agrobacterium-mediated gene transfer system. Immature rice embryos (10-12 days after anthesis) were infected with an Agrobacterium strain carrying a plasmid containing chimeric genes of beta-glucuronidase (uidA) and neomycin phosphotransferase (nptII). Co-incubation of potato suspension culture (PSC) with the Agrobacterium inoculum significantly improved the transformation efficiency of rice. The uidA and nptII genes, which are under the control of promoters of a rice alpha-amylase gene (alphaAmy8) and Agrobacterium nopaline synthase gene (nos), respectively, were both expressed in G418-resistant calli and transgenic plants. Integration of foreign genes into the genomes of transgenic plants was confirmed by Southern blot analysis. Histochemical localization of GUS activity in one transgenic plant (RO) revealed that the rice alpha-amylase promoter functions in all cell types of the mature leaves, stems, sheaths and roots, but not in the very young leaves. This transgenic plant grew more slowly and produced less seeds than the wild-type plant, but its R1 and R2 progenies grew normally and produced as much seeds as the wild-type plant. Inheritance of foreign genes to the progenies was also confirmed by Southern blot analysis. These data demonstrate successful gene transfer and sexual inheritance of the chimeric genes. |
| Document Type: Article |
| Language: English |
Addresses:
1. ACAD SINICA, INST MOLEC BIOL, TAIPEI 11529, TAIWAN
2. NATL TAIWAN UNIV, DEPT AGRON, TAIPEI 10764, TAIWAN
3. NATL NORMAL UNIV, DEPT BIOL, TAIPEI 11718, TAIWAN |
| Publisher: KLUWER ACADEMIC PUBL, SPUIBOULEVARD 50, PO BOX 17, 3300 AA DORDRECHT, NETHERLANDS |
| Subject Category: Biochemistry & Molecular Biology; Plant Sciences |
| IDS Number: LL038 |
| ISSN: 0167-4412 |
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