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| INHIBITION OF ADENYLYL-CYCLASE BY G(I-ALPHA) |
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| Author(s): TAUSSIG R, INIGUEZLLUHI JA, GILMAN AG |
| Source: SCIENCE Volume: 261 Issue: 5118 Pages: 218-221 Published: JUL 9 1993 |
| Times Cited: 244 References: 31 |
| Abstract: Evidence suggests that both alpha and betagamma subunits of heterotrimeric guanine nucleotide-binding regulatory proteins (G proteins) inhibit adenylyl cyclase. Although type I adenylyl cyclase is inhibited directly by exogenous betagamma, inhibition of adenylyl cyclase by G(ialpha) has not been convincingly demonstrated in vitro. Concentration-dependent inhibition of adenylyl cyclases by purified G(ialpha) subunits is described. Activated G(ialpha) but not G(oalpha) was effective, and myristoylation of G(ialpha) was required. The characteristics of the inhibitory effect were dependent on the type of adenylyl cyclase and the nature of the activator of the enzyme. The concentrations of G(ialpha) required to inhibit adenylyl cyclase were substantially higher than those normally thought to be relevant physiologically. However, analysis indicates that these concentrations may be relevant and reasonable. |
| Document Type: Article |
| Language: English |
| Reprint Address: TAUSSIG, R (reprint author), UNIV TEXAS, SW MED CTR, DEPT PHARMACOL, 5323 HARRY HINES BLVD, DALLAS, TX 75235 USA |
| Publisher: AMER ASSOC ADVANCEMENT SCIENCE, 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: LL596 |
| ISSN: 0036-8075 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |