| DNA-DEPENDENT AND RNA-DEPENDENT DNA-POLYMERASES |
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| Author(s): STEITZ TA |
| Source: CURRENT OPINION IN STRUCTURAL BIOLOGY Volume: 3 Issue: 1 Pages: 31-38 Published: FEB 1993 |
| Times Cited: 154 References: 33 |
| Abstract: A catalytic subdomain of HIV reverse transcriptase has the same structure as that of E. coli Klenow fragment, including three conserved carboxylates whose mutation destroys activity. The structures of Klenow fragment complexed with DNA and with divalent metal ions, taken together with mutagenic studies, are consistent with a two-metal-ion mechanism of phosphoryl transfer at both active sites. The source of processivity in DNA polymerase III is easily understood in terms of the donut shape of its beta-subunit. |
| Document Type: Article |
| Language: English |
Addresses:
1. YALE UNIV, NEW HAVEN, CT 06520 USA |
| Publisher: CURRENT BIOLOGY LTD, 34-42 CLEVELAND STREET, LONDON, ENGLAND W1P 6LB |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology |
| IDS Number: LU404 |
| ISSN: 0959-440X |