| | |  | | | | Record from Web of Science® | | | | | | |
| IL-1-ALPHA INHIBITS HUMAN HAIR FOLLICLE GROWTH AND HAIR FIBER PRODUCTION IN WHOLE-ORGAN CULTURES |
|
|
| Author(s): HARMON CS, NEVINS TD |
| Source: LYMPHOKINE AND CYTOKINE RESEARCH Volume: 12 Issue: 4 Pages: 197-203 Published: AUG 1993 |
| Times Cited: 61 References: 46 |
| Abstract: In this study we have used a recently developed human hair follicle whole-organ culture system to investigate the effect of IL-1alpha on hair follicle growth and hair fiber production. In the presence of 10 ng/ml IL-1alpha, the growth of cultured human hair follicles ceased within 2-4 days, whereas control hair follicles grew for a period of 7-10 days. IL-1alpha also inhibited hair fiber growth, but with an onset which occurred 3 days later than that of follicle growth inhibition. An IC50 value of approximately 30 pg/ml was obtained for IL-1alpha inhibition of follicle growth Incubation of hair follicles with IL-1alpha resulted in a rapid, transient reduction in the rate of whole-follicle DNA synthesis. 1000-fold molar excess of IL-1 receptor antagonist prevented IL-1-induced follicle growth inhibition, while antagonist alone was without effect. The selective PKC inhibitor, Ro 31-7549, augmented IL-1-induced inhibition of hair follicle growth, but did not itself affect hair follicle growth. These findings indicate that IL-1alpha exerts a rapid antiproliferative effect on hair follicles, and that inhibition of hair fiber growth is a secondary response. Thus, IL-1 may play a role in the pathophysiology of inflammatory hair loss conditions, such as alopecia areata, through a direct growth-inhibitory effect on hair follicles. |
| Document Type: Article |
| Language: English |
| Reprint Address: HARMON, CS (reprint author), HOFFMANN LA ROCHE INC, PRECLIN DERMATOL RES, 86-7 340 KINGSLAND ST, NUTLEY, NJ 07110 USA |
| Publisher: MARY ANN LIEBERT INC PUBL, 2 MADISON AVENUE, LARCHMONT, NY 10538 |
| Subject Category: Biochemistry & Molecular Biology; Immunology |
| IDS Number: LU617 |
| ISSN: 0277-6766 |
|
| | | | | | | | | Record from Web of Science® | | | | | | | | | |