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| INTRODUCTION AND EXPRESSION OF THE 400 KILOBASE PRECURSOR AMYLOID PROTEIN GENE IN TRANSGENIC MICE |
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| Author(s): LAMB BT, SISODIA SS, LAWLER AM, SLUNT HH, KITT CA, KEARNS WG, PEARSON PL, PRICE DL, GEARHART JD |
| Source: NATURE GENETICS Volume: 5 Issue: 1 Pages: 22-29 Published: SEP 1993 |
| Times Cited: 200 References: 67 |
| Abstract: Overexpression of the gene encoding the beta-amyloid precursor protein (APP) may have a key role in the pathogenesis of both Alzheimer's disease (AD) and Down Syndrome (DS). We have therefore introduced a 650 kilobase (kb) yeast artificial chromosome (YAC) that contains the entire, unrearranged 400 kb human APP gene into mouse embryonic stem (ES) cells by lipid-mediated transfection. ES lines were generated that contain a stably integrated, unrearranged human APP gene. Moreover, we demonstrate germ line transmission of the APP YAC in transgenic mice and expression of human APP mRNA and protein at levels comparable to endogenous APP. This transgenic strategy may prove invaluable for the development of mouse models for AD and DS. |
| Document Type: Article |
| Language: English |
Addresses:
1. JOHNS HOPKINS UNIV, SCH MED, DEPT GYNECOL & OBSTET, DEV GENET LAB, 205 PHYSIOL BLDG, 725 N WOLFE ST, BALTIMORE, MD 21205 USA
2. JOHNS HOPKINS UNIV, SCH MED, DEPT PATHOL, NEUROPATHOL LAB, BALTIMORE, MD 21205 USA
3. JOHNS HOPKINS UNIV, SCH MED, CTR MED GENET, DEPT MED, BALTIMORE, MD 21205 USA |
| Publisher: NATURE PUBLISHING CO, 345 PARK AVE SOUTH, NEW YORK, NY 10010-1707 |
| Subject Category: Genetics & Heredity |
| IDS Number: LV565 |
| ISSN: 1061-4036 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |