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| UNIVERSAL AMPLIFICATION AND ANALYSIS OF PATHOGEN 16S RDNA FOR CLASSIFICATION AND IDENTIFICATION OF MYCOPLASMALIKE ORGANISMS |
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| Author(s): LEE IM, HAMMOND RW, DAVIS RE, GUNDERSEN DE |
| Source: PHYTOPATHOLOGY Volume: 83 Issue: 8 Pages: 834-842 Published: AUG 1993 |
| Times Cited: 323 References: 22 |
| Abstract: Regions representing about 80% of the 16S rDNA sequences of 40 mycoplasmalike organism (MLO) strains from North America, Asia, and Europe were amplified by polymerase chain reaction (PCR) using a primer pair designed on the basis of an MLO 16S rRNA gene. This primer pair detected every MLO examined from infected periwinkle (Catharanthus roseus) and some other plants. No PCR products were obtained in samples containing DNA extracted from healthy plants. The partial 16S rDNA sequences amplified from these various MLOs were compared through restriction fragment length polymorphism (RFLP) analyses. Based on similarity coefficients derived from RFLP analyses, these 40 MLOs could be classified into nine distinct 16S ribosomal RNA (16Sr) groups and 14 subgroups, including five groups that coincide with MLO strain clusters previously delineated on the basis of dot hybridization analysis using randomly cloned chromosomal DNA probes. Type MLO strains designated for each group and subgroup were as follows: 16SrI-A, tomato big bud; 16SrI-B, Maryland aster yellows; 16SrI-C, clover phyllody; 16SrI-D, paulownia witches'-broom; 16SrI-E, blueberry stunt; 16SrII, peanut witches'-broom; 16SrIII-A, Canada peach X; 16SrIII-B, clover yellow edge; 16SrIV, palm lethal yellowing; 16SrV, elm yellows; 16SrVI, clover proliferation; 16SrVII, ash yellows; 16SrVIII, loofah witches'-broom; and 16SrIX, pigeon pea witches-broom. |
| Document Type: Article |
| Language: English |
| Reprint Address: LEE, IM (reprint author), USDA ARS, BELTSVILLE AGR RES CTR, MOLEC PLANT PATHOL LAB, BELTSVILLE, MD 20705 USA |
Addresses:
1. GEORGE WASHINGTON UNIV, MED CTR, DEPT MICROBIOL & IMMUNOL, WASHINGTON, DC 20037 USA |
| Publisher: AMER PHYTOPATHOLOGICAL SOC, 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 |
| Subject Category: Plant Sciences |
| IDS Number: LW495 |
| ISSN: 0031-949X |
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