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| SCREENING PATIENTS FOR HETEROZYGOUS-P53 MUTATIONS USING A FUNCTIONAL ASSAY IN YEAST |
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| Author(s): ISHIOKA C, FREBOURG T, YAN YX, VIDAL M, FRIEND SH, SCHMIDT S, IGGO R |
| Source: NATURE GENETICS Volume: 5 Issue: 2 Pages: 124-129 Published: OCT 1993 |
| Times Cited: 190 References: 43 |
| Abstract: Inherited mutations of the p53 gene significantly increase the risk of developing diverse malignancies, and germline p53 mutations can be detected by assaying the transcriptional activity of the p53 protein in mammalian cells. Here we describe a method starting with lymphocytes that allows detection of germline p53 mutations by 'functional' analysis of p53 protein expressed in Saccharomyces cerevisiae. The p53 PCR products are directly cloned into yeast expression vectors in vivo and subsequently tested for transcriptional activity in a simple growth assay. This technique, functional analysis of separated alleles in yeast (FASAY), requires only a few steps, can be automated readily and should permit screening for germline or somatic heterozygous mutations in any gene whose function can be monitored in yeast. |
| Document Type: Article |
| Language: English |
Addresses:
1. MASSACHUSETTS GEN HOSP, CTR CANC, BOSTON, MA 02129 USA
2. TOHOKU UNIV, INST DEV AGING & CANC, DEPT CLIN ONCOL, SENDAI, MIYAGI 980 JAPAN
3. HARVARD UNIV, CHILDRENS HOSP, SCH MED, DANA FARBER INST, DEPT PEDIAT, DW HEMATOL ONCOL, BOSTON, MA 02114 USA
4. SWISS INST EXPTL CANC RES, CH-1066 EPALINGES, SWITZERLAND |
| Publisher: NATURE PUBLISHING CO, 345 PARK AVE SOUTH, NEW YORK, NY 10010-1707 |
| Subject Category: Genetics & Heredity |
| IDS Number: MA056 |
| ISSN: 1061-4036 |
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