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| IMPROVED GENE-TRANSFER BY DIRECT PLASMID INJECTION ASSOCIATED WITH REGENERATION IN MOUSE SKELETAL-MUSCLE |
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| Author(s): WELLS DJ |
| Source: FEBS LETTERS Volume: 332 Issue: 1-2 Pages: 179-182 Published: OCT 11 1993 |
| Times Cited: 107 References: 30 |
| Abstract: Gene transfer into skeletal muscle via simple plasmid injection in vivo has many potential uses but these are severely constrained by the low efficiency of this technique. Muscle regeneration, induced by the myotoxic local anaesthetic bupivacaine, significantly increased gene expression following plasmid injection 3-7 days after bupivacaine treatment. Much of this effect can be attributed to uptake and expression of the plasmid by a greater number of muscle fibres, up to 9% of the mouse tibialis anterior muscle. A similar significant increase in expression was observed in the naturally regenerating muscle of the dystropbic mdx mouse when compared to the control C57B1/10 strain. |
| Document Type: Article |
| Language: English |
| Reprint Address: WELLS, DJ (reprint author), UNIV LONDON ROYAL VET COLL, DEPT VET BASIC SCI, ROYAL COLL ST, LONDON NW1 0TU, ENGLAND |
| Publisher: ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS |
| Subject Category: Biochemistry & Molecular Biology; Biophysics; Cell Biology |
| IDS Number: MA778 |
| ISSN: 0014-5793 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |