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| IDENTIFICATION OF A 120 KD HAIR-BUNDLE MYOSIN LOCATED NEAR STEREOCILIARY TIPS |
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| Author(s): GILLESPIE PG, WAGNER MC, HUDSPETH AJ |
| Source: NEURON Volume: 11 Issue: 4 Pages: 581-594 Published: OCT 1993 |
| Times Cited: 116 References: 72 |
| Abstract: By adapting to sustained stimuli, hair cells of the internal ear maintain their optimal sensitivity to minute displacements. Biophysical experiments have suggested that adaptation is mediated by a molecular motor, most likely a member of the myosin family. To provide direct evidence for the presence of myosin isozymes in hair bundles, we used photoaffinity labeling with vanadate-trapped uridine and adenine nucleotides to identify proteins of 120, 160, and 230 kd in a preparation of hair bundles purified from the bullfrog's sacculus. The photoaffinity labeling properties of these proteins, particularly the 120 kd protein, resembled those of other well-characterized myosins. A 120 kd hair-bundle protein was also recognized by a monoclonal antibody directed against a vertebrate myosin I isozyme. Immunofluorescence microscopy localized this protein near the beveled top edge of the hair bundle, the site of mechanoelectrical transduction and adaptation. |
| Document Type: Article |
| Language: English |
Addresses:
1. UNIV TEXAS, SW MED CTR, HOWARD HUGHES MED INST, DALLAS, TX 75235 USA
2. UNIV TEXAS, SW MED CTR, CTR BASIC NEUROSCI RES, DEPT PHARMACOL, DALLAS, TX 75235 USA |
| Publisher: CELL PRESS, 1050 MASSACHUSETTES AVE, CIRCULATION DEPT, CAMBRIDGE, MA 02138 |
| Subject Category: Neurosciences |
| IDS Number: MD068 |
| ISSN: 0896-6273 |
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