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| THE KINETICS OF SYNAPTIC VESICLE RECYCLING MEASURED AT SINGLE PRESYNAPTIC BOUTONS |
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| Author(s): RYAN TA, REUTER H, WENDLAND B, SCHWEIZER FE, TSIEN RW, SMITH SJ |
| Source: NEURON Volume: 11 Issue: 4 Pages: 713-724 Published: OCT 1993 |
| Times Cited: 346 References: 27 |
| Abstract: We used the fluorescent membrane probe FM 1-43 to label recycling synaptic vesicles within the presynaptic boutons of dissociated hippocampal neurons in culture. Quantitative time-lapse fluorescence imaging was employed in combination with rapid superfusion techniques to study the dynamics of synaptic vesicles within single boutons. This approach enabled us to measure exocytosis and to analyze the kinetics of endocytosis and the preparation of endocytosed vesicles for re-release (repriming). Our measurements indicate that under sustained membrane depolarization, endocytosis persists much longer than exocytosis, with a t1/2 almost-equal-to 60 s (approximately 24-degrees-C); once internalized, vesicles become reavailable for exocytosis in approximately 30 s. Furthermore, we have shown that endocytosis is not dependent on membrane potential and, unlike exocytosis, that it is independent of extracellular Ca2+. |
| Document Type: Article |
| Language: English |
| Reprint Address: RYAN, TA (reprint author), STANFORD UNIV, DEPT MOLEC & CELLULAR PHYSIOL, STANFORD, CA 94305 USA |
Addresses:
1. UNIV BERN, DEPT PHARMACOL, CH-3010 BERN, SWITZERLAND |
| Publisher: CELL PRESS, 1050 MASSACHUSETTES AVE, CIRCULATION DEPT, CAMBRIDGE, MA 02138 |
| Subject Category: Neurosciences |
| IDS Number: MD068 |
| ISSN: 0896-6273 |
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