| RAPID TRANSGENE EXPRESSION IN LYMPHOCYTE AND MACROPHAGE PRIMARY CULTURES AFTER PARTICLE BOMBARDMENT-MEDIATED GENE-TRANSFER |
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| Author(s): BURKHOLDER JK, DECKER J, YANG NS |
| Source: JOURNAL OF IMMUNOLOGICAL METHODS Volume: 165 Issue: 2 Pages: 149-156 Published: OCT 15 1993 |
| Times Cited: 62 References: 19 |
| Abstract: A particle bombardment technique was used for gene transfer to human peripheral blood mononuclear cells, and murine splenocytes, thymocytes and peritoneal macrophages in primary culture. Significant expression of a luciferase marker gene was observed in these cell types within 8 h of gene transfer. Luciferase expression was readily detected in peritoneal macrophages 4 h after culture initiation and transfection. Same day determinations of transgene activity in fresh human peripheral blood mononuclear cell samples were feasible. Promoter preference and ballistic parameters were examined to optimize transgene expression. Up to 6% of bombarded human T lymphocytes expressed transgenic beta-galactosidase activity. These results demonstrate that particle bombardment is an effective means for gene transfer and provides an attractive approach for rapid, quantitative analysis of transgene expression in various leukocyte primary culture systems. |
| Document Type: Article |
| Language: English |
Addresses:
1. AGRACETUS INC, DEPT MAMMALIAN GENET, 8520 UNIV GREEN, MIDDLETON, WI 53562 USA |
| Publisher: ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS |
| Subject Category: Biochemical Research Methods; Immunology |
| IDS Number: MD377 |
| ISSN: 0022-1759 |