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| EXPRESSION OF MESSENGER-RNA FOR NEUROTROPHIC FACTORS AND THEIR RECEPTORS IN THE RAT DORSAL-ROOT GANGLION AND SCIATIC-NERVE FOLLOWING NERVE INJURY |
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| Author(s): SEBERT ME, SHOOTER EM |
| Source: JOURNAL OF NEUROSCIENCE RESEARCH Volume: 36 Issue: 4 Pages: 357-367 Published: NOV 1 1993 |
| Times Cited: 141 References: 56 |
| Abstract: The adult rat dorsal root ganglion (DRC) produces mRNA for the neurotrophic factors nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) and contains large populations of neurons responsive to these factors. We report that following a focal crush injury of the sciatic nerve, NGF mRNA expression increases threefold and BDNF mRNA twofold, in the ipsilateral L4 and L5 DRGs. The mRNAs encoding the cognate neurotrophin receptors, p75NGFR, trkA, and trkB were expressed in the DRC throughout the post-injury time course, suggesting that DRG neurons remain responsive to both NGF and BDNF. p75NGFR mRNA levels became transiently depressed in the DRG during the first several days after the lesion but returned to normal within 1 week. trkB mRNA was expressed in the normal sciatic nerve and levels were not altered by nerve crush. RNase protection assays detected both full-length and truncated trkB transcripts in the DRG, but only truncated trkB mRNA, lacking the tyrosine kinase domain, was detected in the sciatic nerve. Likewise, trkA transcripts were not detected by RNase protection in normal sciatic nerve or in a segment of nerve distal to the crush site. These results are consistent with a model in which regenerating sensory neurons are supported by neurotrophic factors synthesized within the DRG. (C) 1993 Wiley-Liss, Inc. |
| Document Type: Article |
| Language: English |
Addresses:
1. STANFORD UNIV, MED CTR, SCH MED, DEPT NEUROBIOL, STANFORD, CA 94305 USA |
| Publisher: WILEY-LISS, DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 |
| Subject Category: Neurosciences |
| IDS Number: ME272 |
| ISSN: 0360-4012 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |