| | |  | | | | Record from Web of Science® | | | | | | |
| ENHANCEMENT OF AXIAL RESOLUTION IN FLUORESCENCE MICROSCOPY BY STANDING-WAVE EXCITATION |
|
|
| Author(s): BAILEY B, FARKAS DL, TAYLOR DL, LANNI F |
| Source: NATURE Volume: 366 Issue: 6450 Pages: 44-48 Published: NOV 4 1993 |
| Times Cited: 123 References: 27 |
| Abstract: THE use of fluorescence microscopy for investigating the three-dimensional structure of cells and tissue is of growing importance in cell biology, biophysics and biomedicine. Three-dimensional data are obtained by recording a series of images of the specimen as it is stepped through the focal plane of the microscope1-3. Whether by direct imaging or by confocal scanning4,5, diffraction effects and noise generally limit axial resolution to about 0.5 mum. Here we describe a fluorescence microscope in which axial resolution is increased to better than 0.05 mum by using the principle of standing-wave excitation of fluorescence. Standing waves formed by interference in laser illumination create an excitation field with closely spaced nodes and antinodes, allowing optical sectioning of the specimen at very high resolution. We use this technique to obtain images of actin fibres and filaments in fixed cells, actin single filaments in vitro and myosin II in a living cell. |
| Document Type: Article |
| Language: English |
Addresses:
1. CARNEGIE MELLON UNIV, CTR LIGHT MICROSCOPE IMAGING & BIOTECHNOL, 4400 5TH AVE, PITTSBURGH, PA 15213 USA
2. CARNEGIE MELLON UNIV, DEPT BIOL SCI, PITTSBURGH, PA 15213 USA
3. CARNEGIE MELLON UNIV, DEPT PHYS, PITTSBURGH, PA 15213 USA |
| Publisher: MACMILLAN MAGAZINES LTD, PORTERS SOUTH, 4 CRINAN ST, LONDON, ENGLAND N1 9XW |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: MF007 |
| ISSN: 0028-0836 |
|
| | | | | | | | | Record from Web of Science® | | | | | | | | | |