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| PHOTO-CROSS-LINKING OF 5-IODOURACIL SUBSTITUTED RNA AND DNA TO PROTEINS |
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| Author(s): WILLIS MC, HICKE BJ, UHLENBECK OC, CECH TR, KOCH TH |
| Source: SCIENCE Volume: 262 Issue: 5137 Pages: 1255-1257 Published: NOV 19 1993 |
| Times Cited: 138 References: 25 |
| Abstract: 5-Iodouracil-substituted RNA and DNA were crosslinked regiospecifically to associated proteins in yields of 70 to 94% of bound nucleic acid. Irradiation of the iodouracil chromophore with monochromatic, long-wavelength ultraviolet radiation (325 nanometers) eliminates excitation of other nucleic acid and protein chromophores. The combination of high crosslinking yields, excellent specificity, and elimination of photodamage to other chromophores represents an important advance toward the precise identification of contacts in nucleoprotein complexes. |
| Document Type: Article |
| Language: English |
Addresses:
1. UNIV COLORADO, DEPT CHEM & BIOCHEM, BOULDER, CO 80309 USA
2. UNIV COLORADO, HOWARD HUGHES MED INST, DEPT MOLEC CELLULAR & DEV BIOL, BOULDER, CO 80309 USA |
| Publisher: AMER ASSOC ADVANCEMENT SCIENCE, 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: MH324 |
| ISSN: 0036-8075 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |