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| STEPWISE ASSEMBLY OF FUNCTIONALLY ACTIVE-TRANSPORT VESICLES |
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| Author(s): OSTERMANN J, ORCI L, TANI K, AMHERDT M, RAVAZZOLA M, ELAZAR Z, ROTHMAN JE |
| Source: CELL Volume: 75 Issue: 5 Pages: 1015-1025 Published: DEC 3 1993 |
| Times Cited: 238 References: 66 |
| Abstract: Budding of COP-coated vesicles (the likely carriers of newly synthesized proteins from the endoplasmic reticulum through the Golgi stack) from Golgi cisternae requires ADP-ribosylation factor (ARF), coatomer proteins from the cytosol, GTP, and fatty acyl-coenzyme A (CoA). The assembly of coated buds on the membranes requires coatomer, ARF, and GTP. When palmitoyl-CoA is added, membrane fission occurs at the coated bud, releasing coated vesicles. We show that COP-coated vesicles can be generated stepwise in vitro and isolated in a functionally active form, demonstrating that the minimal set of cytosolic components required for their formation as well as principal steps in their assembly have been identified. |
| Document Type: Article |
| Language: English |
| Reprint Address: OSTERMANN, J (reprint author), MEM SLOAN KETTERING CANC CTR, CELLULAR BIOCHEM & BIOPHYS PROGRAM, 1275 YORK AVE, NEW YORK, NY 10021 USA |
Addresses:
1. UNIV GENEVA, SCH MED, INST HISTOL & EMBRYOL, DEPT MORPHOL, CH-1211 GENEVA 4, SWITZERLAND |
| Publisher: CELL PRESS, 1050 MASSACHUSETTES AVE, CIRCULATION DEPT, CAMBRIDGE, MA 02138 |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology |
| IDS Number: MK966 |
| ISSN: 0092-8674 |
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