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| PLASMID DNA IS SUPERIOR TO VIRAL VECTORS FOR DIRECT GENE-TRANSFER INTO ADULT-MOUSE SKELETAL-MUSCLE |
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| Author(s): DAVIS HL, DEMENEIX BA, QUANTIN B, COULOMBE J, WHALEN RG |
| Source: HUMAN GENE THERAPY Volume: 4 Issue: 6 Pages: 733-740 Published: DEC 1993 |
| Times Cited: 250 References: 41 |
| Abstract: Direct gene transfer into skeletal muscle offers several therapeutic possibilities. We assessed direct intramuscular injection of recombinant plasmids, adenovirus, or retrovirus in normal or regenerating muscles of mice. The incorporation and expression of reporter genes introduced by any of these three vectors is greater in regenerating than in mature muscle. In regenerating muscle, pure DNA and adenovirus result in equivalent numbers of fibers expressing reporter gene (>10%), but adenovirus also induces considerable cellular infiltration. In mature muscle, recombinant DNA is better than adenovirus. Retrovirus failed to infect mature muscle fibers and was less effective than plasmid DNA or adenovirus in regenerating muscle. The surprisingly high relative efficiency of pure plasmid DNA suggests that this method will provide a simple, safe and viable alternative for gene therapy involving muscle tissue. |
| Document Type: Article |
| Language: English |
| Reprint Address: DAVIS, HL (reprint author), UNIV OTTAWA, FAC HLTH SCI, PHYSIOTHERAPY PROGRAM, OTTAWA K1H 8M5, ONTARIO CANADA |
Addresses:
1. UNIV OTTAWA, FAC MED, DEPT PHYSIOL, OTTAWA K1H 8M5, ONTARIO CANADA
2. UNIV OTTAWA, FAC MED, DEPT BIOCHEM, OTTAWA K1H 8M5, ONTARIO CANADA
3. MUSEUM NATL HIST NAT, PHYSIOL GEN & COMPAREE LAB, F-75231 PARIS 05, FRANCE
4. INST CHIM BIOL, GEN MOLEC EUCARYOTES LAB, CNRS, INSERM, U184, F-67085 STRASBOURG, FRANCE
5. INST PASTEUR, DEPT BIOL MOLEC, F-75724 PARIS 15, FRANCE |
| Publisher: MARY ANN LIEBERT INC PUBL, 2 MADISON AVENUE, LARCHMONT, NY 10538 |
| Subject Category: Biotechnology & Applied Microbiology; Genetics & Heredity; Medicine, Research & Experimental |
| IDS Number: MM370 |
| ISSN: 1043-0342 |
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