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| MOLECULAR-CLONING AND EXPRESSION OF THE FAS LIGAND, A NOVEL MEMBER OF THE TUMOR-NECROSIS-FACTOR FAMILY |
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| Author(s): SUDA T, TAKAHASHI T, GOLSTEIN P, NAGATA S |
| Source: CELL Volume: 75 Issue: 6 Pages: 1169-1178 Published: DEC 17 1993 |
| Times Cited: 2,067 References: 59 |
| Abstract: The Fas antigen (Fas) belongs to the tumor necrosis factor (TNF)/nerve growth factor receptor family, and it mediates apoptosis. Using a soluble form of mouse Fas, prepared by fusion with human immunoglobulin Fc, Fas ligand was detected on the cell surface of a cytotoxic T cell hybridoma, PC60-d10S. A cell population that highly expresses Fas ligand was sorted using a fluorescence-activated cell sorter, and its cDNA was isolated from the sorted cells by expression cloning. The amino acid sequence indicated that Fas ligand is a type II transmembrane protein that belongs to the TNF family. The recombinant Fas ligand expressed in COS cells induced apoptosis in Fas-expressing target cells. Northern hybridization revealed that Fas ligand is expressed in activated splenocytes and thymocytes, consistent with its involvement in T cell-mediated cytotoxicity and in several nonlymphoid tissues, such as testis. |
| Document Type: Article |
| Language: English |
| Reprint Address: SUDA, T (reprint author), OSAKA BIOSCI INST, 6-2-4 FURUEDAI, SUITA, OSAKA 565 JAPAN |
Addresses:
1. CTR NATL RECH SCI MARSEILLE LUMINY, CTR IMMUNOL, INSERM, F-13288 MARSEILLE 9, FRANCE |
| Publisher: CELL PRESS, 1050 MASSACHUSETTES AVE, CIRCULATION DEPT, CAMBRIDGE, MA 02138 |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology |
| IDS Number: MM893 |
| ISSN: 0092-8674 |
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