| | |  | | | | Record from Web of Science® | | | | | | |
| EXPRESSION CLONING OF A FUNCTIONAL GLYCOPROTEIN LIGAND FOR P-SELECTIN |
|
|
| Author(s): SAKO D, CHANG XJ, BARONE KM, VACHINO G, WHITE HM, SHAW G, VELDMAN GM, BEAN KM, AHERN TJ, FURIE B, CUMMING DA, LARSEN GR |
| Source: CELL Volume: 75 Issue: 6 Pages: 1179-1186 Published: DEC 17 1993 |
| Times Cited: 560 References: 41 |
| Abstract: The initial adhesive interactions between circulating leukocytes and endothelia are mediated, in part, by P-selectin. We now report the expression cloning of a functional ligand for P-selectin from an HL-60 cDNA library. The predicted amino acid sequence reveals a novel mucin-like transmembrane protein. Significant binding of transfected COS cells to P-selectin requires coexpression of both the protein ligand and a fucosyltransferase. This binding is calcium dependent and can be inhibited by a neutralizing monoclonal antibody to P-selectin. Cotransfected COS cells express the ligand as a homodimer of 220 kd. A soluble ligand construct, when coexpressed with fucosyltransferase in COS cells, also mediates P-selectin binding and is immunocrossreactive with the major HL-60 glycoprotein that specifically binds P-selectin. |
| Document Type: Article |
| Language: English |
| Reprint Address: SAKO, D (reprint author), SMALL MOLEC DRUG DISCOVERY GRP, INST GENET, 87 CAMBRIDGE PK DR, CAMBRIDGE, MA 02140 USA |
Addresses:
1. TUFTS UNIV NEW ENGLAND MED CTR, CTR HEMOSTASIS & THROMBOSIS RES, DIV HEMATOL & ONCOL, BOSTON, MA 02111 USA
2. TUFTS UNIV, SCH MED, DEPT MED, BOSTON, MA 02111 USA |
| Publisher: CELL PRESS, 1050 MASSACHUSETTES AVE, CIRCULATION DEPT, CAMBRIDGE, MA 02138 |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology |
| IDS Number: MM893 |
| ISSN: 0092-8674 |
|
| | | | | | | | | Record from Web of Science® | | | | | | | | | |