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| A NEW BACTERIOPHAGE P1-DERIVED VECTOR FOR THE PROPAGATION OF LARGE HUMAN DNA FRAGMENTS |
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| Author(s): IOANNOU PA, AMEMIYA CT, GARNES J, KROISEL PM, SHIZUYA H, CHEN C, BATZER MA, DEJONG PJ |
| Source: NATURE GENETICS Volume: 6 Issue: 1 Pages: 84-89 Published: JAN 1994 |
| Times Cited: 674 References: 46 |
| Abstract: We have designed a P1 vector (pCYPAC-1) for the introduction of recombinant DNA into E coli using electroporation procedures. The new cloning system, P1-derived arteficial chromosomes (PACs), was used to establish an initial 15,000 clone library with an average insert size of 130-150 kilobase pairs (kb). No chimaerism has been observed in 34 clones, by fluorescence in situ hybridization. Similarly, no insert instability has been observed after extended culturing, for 20 clones. We conclude that the PAC cloning system will be useful in the mapping and detailed analysis of complex genomes. |
| Document Type: Article |
| Language: English |
Addresses:
1. ROSWELL PK CANC INST, DEPT HUMAN GENET, ELM & CARLTON ST, BUFFALO, NY 14263 USA
2. LAWRENCE LIVERMORE NATL LAB, CTR HUMAN GENOME L452, BIOL & BIOTECHNOL RES PROGRAM, LIVERMORE, CA 94550 USA
3. CALTECH, DIV BIOL, PASADENA, CA 91125 USA |
| Publisher: NATURE PUBLISHING CO, 345 PARK AVE SOUTH, NEW YORK, NY 10010-1707 |
| Subject Category: Genetics & Heredity |
| IDS Number: MQ177 |
| ISSN: 1061-4036 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |