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| OXIDATIVE INJURY IN REOXYGENATED AND REPERFUSED HEARTS |
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| Author(s): SAMAJA M, MOTTERLINI R, SANTORO F, DELLANTONIO G, CORNO A |
| Source: FREE RADICAL BIOLOGY AND MEDICINE Volume: 16 Issue: 2 Pages: 255-262 Published: FEB 1994 |
| Times Cited: 30 References: 32 |
| Abstract: In this study, we separated the effects of low oxygen supply and low coronary flow in isolated perfused rat hearts to focus on the genesis of free radicals-induced reperfusion injury. Hearts were exposed to either hypoxemia/reoxygenation or ischemia/reperfusion in various sequences, with hypoxemia and ischemia matched for duration (20 min), temperature (37 degrees C), and oxygen supply (10% of baseline). Hypoxemia/reoxygenation (n = 7) resulted in lower (developed pressure) X (heart rate) (p <0.001) and higher end-diastolic pressure (p <0.001) than ischemia/reperfusion (n = 9). The presence of 40 IU/ml superoxide dismutase and 104 IU/ml catalase nearly blunted the rise of the end-diastolic pressure (p = 0.02 vs. baseline), but could only partially prevent the depression of myocardial contractility (p <0.001 vs. baseline, n = 7). Similar patterns were observed when hearts were made ischemic after hypoxemia, eliminating the intermediate reoxygenation step. We conclude that the major determinant of the reperfusion injury is associated with low oxygen supply rather than low coronary flow. Part of the injury is mediated by oxygen-derived free radicals, but a substantial portion of it is associated with energetic processes. |
| Document Type: Note |
| Language: English |
| Reprint Address: SAMAJA, M (reprint author), UNIV MILAN, IST SCI SAN RAFFAELE, DIPARTIMENTO SCI & TECN BIOMED, VIA OLGETTINA 60, I-20132 MILAN, ITALY |
Addresses:
1. UNIV MILAN, IST MALATTIE CARDIOVASC & RESP, I-20132 MILAN, ITALY
2. UNIV MILAN, CATTEDRA ANAT & ISTOL PATOL, I-20132 MILAN, ITALY
3. OSPED SAN DONATO, MILAN, ITALY |
| Publisher: PERGAMON-ELSEVIER SCIENCE LTD, THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB |
| Subject Category: Biochemistry & Molecular Biology; Endocrinology & Metabolism |
| IDS Number: MW774 |
| ISSN: 0891-5849 |
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