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| MULTIMERIC STRUCTURE OF CLC-1 CHLORIDE CHANNEL REVEALED BY MUTATIONS IN DOMINANT MYOTONIA-CONGENITA (THOMSEN) |
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| Author(s): STEINMEYER K, LORENZ C, PUSCH M, KOCH MC, JENTSCH TJ |
| Source: EMBO JOURNAL Volume: 13 Issue: 4 Pages: 737-743 Published: FEB 15 1994 |
| Times Cited: 167 References: 46 |
| Abstract: Voltage-gated ClC chloride channels play important roles in cell volume regulation, control of muscle excitability, and probably transepithelial transport. ClC channels can be functionally expressed without other subunits, but it is unknown whether they function as monomers. We now exploit the properties of human mutations in the muscle chloride channel, ClC-1, to explore its multimeric structure. This is based on analysis of the dominant negative effects of ClC-1 mutations causing myotonia congenita (MC, Thomsen's disease), including a newly identified mutation (P480L) in Thomsen's own family. In a co-expression assay, Thomsen's mutation dramatically inhibits normal ClC-1 function. A mutation found in Canadian MC families (G230E) has a less pronounced dominant negative effect, which can be explained by functional WT/G230E heterooligomeric channels with altered kinetics and selectivity. Analysis of both mutants shows independently that ClC-1 functions as a homeoligomer with most likely four subunits. |
| Document Type: Article |
| Language: English |
Addresses:
1. UNIV HAMBURG, CTR MOLEC NEUROBIOL, D-20246 HAMBURG, GERMANY
2. UNIV MARBURG, HUMAN GENET MED CTR, D-35037 MARBURG, GERMANY |
| Publisher: OXFORD UNIV PRESS UNITED KINGDOM, WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology |
| IDS Number: MX771 |
| ISSN: 0261-4189 |
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