| | |  | | | | Record from Web of Science® | | | | | | |
| NITRIC-OXIDE SYNTHASE ACTIVITY IN HUMAN GYNECOLOGICAL CANCER |
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| Author(s): THOMSEN LL, LAWTON FG, KNOWLES RG, BEESLEY JE, RIVEROSMORENO V, MONCADA S |
| Source: CANCER RESEARCH Volume: 54 Issue: 5 Pages: 1352-1354 Published: MAR 1 1994 |
| Times Cited: 315 References: 19 |
| Abstract: Nitric oxide is generated by the NO synthases, a family of isoenzymes expressed in a wide range of mammalian cells. In the vascular and nervous systems distinct isoforms generate NO to act as a signal transduction mechanism. The isoform induced by cytkines, on the other hand, provides a sustained release of NO which mediates some cytotoxic and cytostatic effects of the immune system. Solid tumors are a heterogeneous population of cell types, including tumor, vascular and infiltrating immune cells. Studies in vitro show that NO synthase can be present in many of these cells. However, its presence in situ in solid human tumors has not been reported. In this study, we have investigated NO synthase activity and its cellular localization in malignant and nonmalignant human gynecological tissue. Nitric oxide synthase activity was observed in malignant tissue, was highest (greater than or equal to 250 pmol/min/g tissue) in poorly differentiated tumors, and was below detectable levels in normal gynecological tissue. Furthermore, investigations with a polyclonal NO sgnthase antibody revealed immunoreactivity only in malignant tissue. This was associated with NO synthase activity and localized to tumor cells. Thus NO synthase is present in human gynecological tumors, and its presence seems to correlate inversely with the differentiation of the tumor. |
| Document Type: Article |
| Language: English |
| Reprint Address: THOMSEN, LL (reprint author), WELLCOME RES LABS, LANGLEY COURT, BECKENHAM BR3 3BS, KENT ENGLAND |
Addresses:
1. KINGS COLL HOSP, DEPT OBSTET & GYNAECOL, LONDON, ENGLAND |
| Publisher: AMER ASSOC CANCER RESEARCH, PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 |
| Subject Category: Oncology |
| IDS Number: MZ092 |
| ISSN: 0008-5472 |
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