| | |  | | | | Record from Web of Science® | | | | | | |
| IDX-1 - A NEW HOMEODOMAIN TRANSCRIPTION FACTOR EXPRESSED IN RAT PANCREATIC-ISLETS AND DUODENUM THAT TRANSACTIVATES THE SOMATOSTATIN GENE |
|
|
| Author(s): MILLER CP, MCGEHEE RE, HABENER JF |
| Source: EMBO JOURNAL Volume: 13 Issue: 5 Pages: 1145-1156 Published: MAR 1 1994 |
| Times Cited: 317 References: 64 |
| Abstract: We describe the cloning from a rat islet somatostatin-producing cell line of a 1.4 kb cDNA encoding a new homeoprotein, IDX-1 (islet/duodenum homeobox-1), with close sequence similarity to the Drosophila melanogaster homeobox protein Antennapedia (Antp) and the Xenopus laevis endoderm-specific homeoprotein XlHbox8. Analyses of IDX-1 mRNA and protein in rat tissues show that IDX-1 is expressed in pancreatic islets and ducts and in the duodenum. In electrophoretic mobility shift assays ZDX-1 binds to three sites in the 5' flanking region of the rat somatostatin gene. In co-transfection experiments IDX-1 transactivates reporter constructs containing somatostatin promoter sequences, and mutation of the IDX-1 binding sites attenuates transactivation. Reverse transcription- polymerase chain reaction of islet RNA using degenerate amplimers for mRNAs encoding homeoproteins indicates that IDX-1 is the most abundant of 12 different Antp-like homeodomain mRNAs expressed in adult rat islets. The pattern of expression, relative abundance and transcriptional regulatory activity suggests that IDX-1 may be involved in the regulation of islet hormone genes and in cellular differentiation in the endocrine pancreas and the duodenum. |
| Document Type: Article |
| Language: English |
| Reprint Address: MILLER, CP (reprint author), HARVARD UNIV, MASSACHUSETTS GEN HOSP, SCH MED, MOLEC ENDOCRINOL LAB, BOSTON, MA 02114 USA |
Addresses:
1. HARVARD UNIV, SCH MED, HOWARD HUGHES MED INST, BOSTON, MA 02114 USA |
| Publisher: OXFORD UNIV PRESS UNITED KINGDOM, WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology |
| IDS Number: NA288 |
| ISSN: 0261-4189 |
|
| | | | | | | | | Record from Web of Science® | | | | | | | | | |