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| PHYSICAL MAPPING OF CHROMOSOME-12Q BREAKPOINTS IN LIPOMA, PLEOMORPHIC SALIVARY-GLAND ADENOMA, UTERINE LEIOMYOMA, AND MYXOID LIPOSARCOMA |
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| Author(s): SCHOENMAKERS EFPM, KOOLS PFJ, MOLS R, KAZMIERCZAK B, BARTNITZKE S, BULLERDIEK J, DALCIN P, DEJONG PJ, VANDENBERGHE H, VANDEVEN WJM |
| Source: GENOMICS Volume: 20 Issue: 2 Pages: 210-222 Published: MAR 15 1994 |
| Times Cited: 58 References: 56 |
| Abstract: We report here the physical mapping of recurrent chromosome 12q13-q15 breakpoints in cell lines derived from primary myxoid liposarcoma, lipoma, uterine leiomyoma, and pleomorphic adenoma of the salivary glands. In fluorescence in situ hybridization (FISH) experiments, we first mapped the position of the chromosome 12 translocation breakpoint in uterine leiomyoma cell line LM-30.1/SV40 relative to loci COL2A1, D12S4, D12S17, D12S6, D12S19, D12S8, and D12S7. It mapped between linkage probes CRI-C86 (D12S19) and p7G11 (D12S8). We then isolated YAC clones using CRI-C86- and p7G11-derived sequence-tagged sites, constructed corresponding YAC contigs of 310 and 800 kb, respectively, and established long-range physical maps of these. Cosmid clones LLNL12NCO1-98C10 and LLNL12NCO1-113D12 were isolated using STSs within the CRI-C86- and the p7G11 -derived YAC contigs, respectively, and a mixture of them was used to routinely study the various tumor cell lines by FISH analysis. The chromosome 12 breakpoints of all tumor cell lines tested mapped between cosmids LLNL12NCO1-98C10 and LLNL12NCO1-113D12. None of the breakpoints appeared to map within any of the isolated YAC clones. Furthermore, FISH analysis using cosmid LLNL12NCO1-144G3, which maps at the CHOP locus, revealed that the chromosome 12 breakpoints in all cell lines of the three benign solid tumors that were tested were located distal to the chromosome 12 translocation breakpoint with the CHOP gene in myxoid liposarcoma cells with t(12;16). In conclusion, our studies seem to indicate that the chromosome 12 breakpoints of myxoid liposarcoma, lipoma, uterine leiomyoma, and pleomorphic adenoma of the salivary glands are all clustered within the 7-cM interval between D12S19 and D12S8, with those of the benign solid tumors distal to CHOP. Finally, the MYF5 gene mapped telomeric to LLNL12NCO1-113D12, and the MIP gene mapped centromeric to the chromosome 12 translocation breakpoint in myxoid liposarcoma cells. (C) 1994 Academic Press, Inc. |
| Document Type: Article |
| Language: English |
| Reprint Address: VANDEVEN, WJM (reprint author), CATHOLIC UNIV LEUVEN, CTR HUMAN GENET, HERESTR 49, B-3000 LOUVAIN, BELGIUM |
Addresses:
1. UNIV BREMEN, CTR HUMAN GENET, D-28359 BREMEN, GERMANY
2. NEW YORK STATE DEPT HLTH, ROSWELL PK MEM INST, BUFFALO, NY 14263 USA |
| Publisher: ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS, 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 |
| Subject Category: Biotechnology & Applied Microbiology; Genetics & Heredity |
| IDS Number: NB820 |
| ISSN: 0888-7543 |
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