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| AN RNA POLYMERASE-II HOLOENZYME RESPONSIVE TO ACTIVATORS |
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| Author(s): KOLESKE AJ, YOUNG RA |
| Source: NATURE Volume: 368 Issue: 6470 Pages: 466-469 Published: MAR 31 1994 |
| Times Cited: 492 References: 30 |
| Abstract: RNA POLYMERASE II requires multiple general transcription factors to initiate site-specific transcription1-3. These proteins can assemble in an ordered fashion onto promoter DNA in vitro2-8, and such ordered assembly may occur in vivo (Fig. 1a). Some general transcription factors can interact with RNA polymerase II in the absence of DNA3,9-15, however, suggesting that RNA polymerase II may also assemble into a multi-component complex containing a subset of initiation factors before binding to promoter DNA (Fig. 1b). Here we present evidence from the yeast Saccharomyces cerevisiae for such an RN.A polymerase II holoenzyme, a multi-subunit complex containing roughly equimolar amounts of RNA polymerase II, a subset of general transcription factors, and SRB regulatory proteins. Transcription by this holoenzyme is stimulated by the activator protein GAL4-VP16, a feature not observed with purified RNA polymerase II and general transcription factors alone. We propose that the holoenzyme is a form of RNA polymerase II readily recruited to promoters in vivo. |
| Document Type: Article |
| Language: English |
| Reprint Address: KOLESKE, AJ (reprint author), WHITEHAD INST BIOMED RES, 9 CAMBRIDGE CTR, CAMBRIDGE, MA 02142 USA |
Addresses:
1. MIT, DEPT BIOL, CAMBRIDGE, MA 02139 USA |
| Publisher: MACMILLAN MAGAZINES LTD, PORTERS SOUTH, 4 CRINAN ST, LONDON, ENGLAND N1 9XW |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: ND120 |
| ISSN: 0028-0836 |
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