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| THE HUMAN GLUCAGON RECEPTOR ENCODING GENE - STRUCTURE, CDNA SEQUENCE AND CHROMOSOMAL LOCALIZATION |
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| Author(s): LOK S, KUIJPER JL, JELINEK LJ, KRAMER JM, WHITMORE TE, SPRECHER CA, MATHEWES S, GRANT FJ, BIGGS SH, ROSENBERG GB, SHEPPARD PO, OHARA PJ, FOSTER DC, KINDSVOGEL W |
| Source: GENE Volume: 140 Issue: 2 Pages: 203-209 Published: MAR 25 1994 |
| Times Cited: 91 References: 33 |
| Abstract: Characterization of the human glucagon-receptor-encoding gene (GGR) should provide a greater understanding of blood glucose regulation and may reveal a genetic basis for the pathogenesis of diabetes. A cDNA encoding a complete functional human glucagon receptor (GGR) was isolated from a liver cDNA library by a combination of polymerase chain reaction and colony hybridization. The cDNA encodes a receptor protein with 80% identity to rat GGR that binds [I-125]glucagon and transduces a signal leading to increases in the concentration of intracellular cyclic adenosine 3',5'-monophosphate. Southern blot analysis of human DNA reveals a hybridization pattern consistent with a single GGR locus. In situ hybridization to metaphase chromosome preparations maps the GGR locus to chromosome 17q25. Analysis of the genomic sequence shows that the coding region spans over 5.5 kb and is interrupted by 12 introns. |
| Document Type: Article |
| Language: English |
| Reprint Address: LOK, S (reprint author), ZYMOGENET INC, DEPT MOLEC & CELLULAR BIOL, 4225 ROOSEVELT WAY NE, SEATTLE, WA 98105 USA |
Addresses:
1. ZYMOGENET INC, DEPT MOLEC PHARMACOL, SEATTLE, WA 98105 USA
2. ZYMOGENET INC, DEPT DNA CHEM & COMP SCI, SEATTLE, WA 98105 USA
3. ZYMOGENET INC, DEPT PROT CHEM, SEATTLE, WA 98105 USA |
| Publisher: ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS |
| Subject Category: Genetics & Heredity |
| IDS Number: ND699 |
| ISSN: 0378-1119 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |