| A PEPTIDE ENCODING THE C-JUN DELTA-DOMAIN INHIBITS THE ACTIVITY OF A C-JUN AMINO-TERMINAL PROTEIN-KINASE |
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| Author(s): ADLER V, UNLAP T, KRAFT AS |
| Source: JOURNAL OF BIOLOGICAL CHEMISTRY Volume: 269 Issue: 15 Pages: 11186-11191 Published: APR 15 1994 |
| Times Cited: 38 References: 20 |
| Abstract: Evidence suggests that the c-Jun protooncogene delta (delta) domain (amino acids 31-60) helps regulate the transcriptional activating capacity of c-Jun by modulating the amino-terminal phosphorylation of this protein. By using a peptide encoding the delta domain and purified amino-terminal c-Jun protein kinase, we demonstrate that the delta domain peptide inhibits phosphorylation of the amino terminus of both c-Jun and the related protein JunD. The delta domain peptide inhibited the activation of the c-Jun amino-terminal protein kinase by phorbol esters in permeabilized U937 leukemic cells. Mutation of c-Jun followed by transfection into U937 leukemic cells demonstrated that partial deletions of the delta domain are sufficient to block phosphorylation of the amino terminus of c-Jun. In vitro deletion of the amino-terminal (amino acids 31-44) half of the delta domain inhibited the phosphorylation of c-Jun. However, deletion of the carboxyl-terminal (amino acids 45-60) half only partially inhibited c-Jun phosphorylation. Therefore, these results indicate that the delta domain sequence is an important regulator of c-Jun amino-terminal phosphorylation. |
| Document Type: Article |
| Language: English |
Addresses:
1. UNIV ALABAMA, DIV HEMATOL ONCOL, BIRMINGHAM, AL 35294 USA |
| Publisher: AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 |
| Subject Category: Biochemistry & Molecular Biology |
| IDS Number: NF966 |
| ISSN: 0021-9258 |