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| HIGH-RESOLUTION ORDERING OF YAC CONTIGS USING EXTENDED CHROMATIN AND CHROMOSOMES |
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| Author(s): HAAF T, WARD DC |
| Source: HUMAN MOLECULAR GENETICS Volume: 3 Issue: 4 Pages: 629-633 Published: APR 1994 |
| Times Cited: 52 References: 13 |
| Abstract: The general usefulness of fluorescence in situ hybridization (FISH) for the physical mapping of the human genome is greatly enhanced by improved DNA resolution. Several techniques have been described for decondensing or stretching interphase chromatin in a linear fashion, allowing long-range FISH mapping in finer detail. Highly extended linear chromatin can be hybridized over physical distances of at least several megabases, possibly over the whole length of a chromosome. By multi-color FISH, we have determined the order and overlaps of YACs from a 5q34 - 35 contig. Cosmids can be localized within larger YAC clones. Extended chromatin mapping can be applied as an adjunct ordering technique in genome studies. |
| Document Type: Article |
| Language: English |
| Reprint Address: HAAF, T (reprint author), YALE UNIV, SCH MED, DEPT GENET, 333 CEDAR ST, POB 3333, NEW HAVEN, CT 06510 USA |
| Publisher: OXFORD UNIV PRESS UNITED KINGDOM, WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP |
| Subject Category: Biochemistry & Molecular Biology; Genetics & Heredity |
| IDS Number: NH456 |
| ISSN: 0964-6906 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |