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| HETEROGENEITY IN THE PROPERTIES OF THE TRYPANOLYTIC FACTOR IN NORMAL HUMAN SERUM |
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| Author(s): LORENZ P, JAMES RW, OWEN JS, BETSCHART B |
| Source: MOLECULAR AND BIOCHEMICAL PARASITOLOGY Volume: 64 Issue: 1 Pages: 153-164 Published: MAR 1994 |
| Times Cited: 22 References: 39 |
| Abstract: Although it seems clear that the trypanolytic factor in human serum capable of killing Trypanosoma brucei brucei is high density lipoprotein (HDL), it nevertheless remains controversial as to whether the trypanolytic properties of HDL are confined to a specific subclass or whether all particles have activity. In the present study, we have compared the lytic activities of serum fractions from six normal individuals prepared by gradient ultracentrifugation and also, to avoid ultracentrifugally-induced loss of HDL apolipoproteins, by gel filtration using fast protein liquid chromatography (FPLC). All sera displayed trypanolytic activity in fractions corresponding to the general density (rho = 1.06-1.20 g ml(-1)) and size (59-440 kDa) limits conventionally used to describe bulk human HDL, the particles between rho = 1.18-1.20 g ml(-1) and between 214-440 kDa being particularly lytic. But some sera additionally contained fractions with powerful activity outside these density (rho > 1.24 g ml(-1)) and size (> 1000 kDa) ranges. Nevertheless, such fractions were considered to contain material with HDL characteristics; apolipoprotein A-I, the major protein of HDL, was always present and the lytic activity of the sera could be completely neutralized by absorption with HDL antiserum. We conclude that all of the trypanolytic activity in human sera is associated with HDL particles and that it is a property of several HDL subpopulations with very different density and size characteristics. Presumably the well-recognized wide variation in trypanocidal activity of normal human sera reflects differences in the quantities of these HDL subpopulations rather than in the total amount of a single, uniquely lytic particle. |
| Document Type: Article |
| Language: English |
| Reprint Address: LORENZ, P (reprint author), SWISS TROP INST, CH-4002 BASEL, SWITZERLAND |
Addresses:
1. HOP CANTONAL GENEVA, DEPT MED, DIV DIABETOL, CH-1211 GENEVA, SWITZERLAND
2. UNIV LONDON, ROYAL FREE HOSP, SCH MED, DEPT MED, LONDON NW3 2PF, ENGLAND |
| Publisher: ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS |
| Subject Category: Biochemistry & Molecular Biology; Parasitology |
| IDS Number: NH707 |
| ISSN: 0166-6851 |
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