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| MOLECULAR-CLONING AND FUNCTIONAL ANAL OF THE ADENOVIRUS E1A-ASSOCIATED 300-KD PROTEIN (P300) REVEALS A PROTEIN WITH PROPERTIES OF A TRANSCRIPTIONAL ADAPTER |
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| Author(s): ECKNER R, EWEN ME, NEWSOME D, GERDES M, DECAPRIO JA, LAWRENCE JB, LIVINGSTON DM |
| Source: GENES & DEVELOPMENT Volume: 8 Issue: 8 Pages: 869-884 Published: APR 15 1994 |
| Times Cited: 774 References: 72 |
| Abstract: The growth-controlling functions of the adenovirus E1A oncoprotein depend on its ability to interact with a set of cellular proteins. Among these are the retinoblastoma protein, p107, p130, and p300. We have isolated a cDNA encoding full-length human p300 and mapped the chromosomal location of the gene to chromosome 22q13. p300 contains three cysteine- and histidine-rich regions of which the most carboxy-terminal region interacts specifically with E1A. In its center, p300 contains a bromodomain, a hallmark of certain transcriptional coactivators. We have examined the ability of p300 to overcome the repressive effect of E1A on the SV40 enhancer. We show that p300 molecules lacking an intact E1A-binding site can bypass E1A repression and restore to a significant extent the activity of the SV40 enhancer, even in the presence of high levels of E1A protein. These results imply that p300 may function as a transcriptional adaptor protein for certain complex transcriptional regulatory elements. |
| Document Type: Article |
| Language: English |
| Reprint Address: ECKNER, R (reprint author), DANA FARBER CANC INST, BOSTON, MA 02115 USA |
Addresses:
1. HARVARD UNIV, SCH MED, BOSTON, MA 02115 USA
2. UNIV MASSACHUSETTS, MED CTR, DEPT CELL BIOL, WORCESTER, MA 01655 USA |
| Publisher: COLD SPRING HARBOR LAB PRESS, 1 BUNGTOWN RD, PLAINVIEW, NY 11724 |
| Subject Category: Cell Biology; Developmental Biology; Genetics & Heredity |
| IDS Number: NK756 |
| ISSN: 0890-9369 |
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