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| EXPRESSION CLONING OF A NOVEL ALPHA-1,3-FUCOSYL-TRANSFERASE THAT IS INVOLVED IN BIOSYNTHESIS OF THE SIALYL-LEWIS-X CARBOHYDRATE DETERMINANTS IN LEUKOCYTES |
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| Author(s): SASAKI K, KURATA K, FUNAYAMA K, NAGATA M, WATANABE E, OHTA S, HANAI N, NISHI T |
| Source: JOURNAL OF BIOLOGICAL CHEMISTRY Volume: 269 Issue: 20 Pages: 14730-14737 Published: MAY 20 1994 |
| Times Cited: 293 References: 55 |
| Abstract: The sialyl Lewis x (NeuAc alpha 2-3Gal beta 1-4(Fuc alpha 1-3)Glc-NAc) determinants serve as ligands in the selectin-mediated adhesion of leukocytes to activated endothelium or platelets. In our efforts to identify glycosyltransferases involved in the biosynthesis of those ligands, we achieved expression cloning of a novel human alpha 1,3-fucosyltransferase termed Fuc-TVII from a THP-1 cDNA library by enrichment of the Namalwa cells highly expressing that determinant with a fluorescence-activated cell sorter. Expression of the COOH-terminal catalytic domain of Fuc-TVII showed an alpha 1,3-fucosyltransferase activity for a type II oligosaccharide with a terminal alpha 2,3-linked sialic acid among various accepters, consistent with that in vivo acceptor specificity. Alignment of the primary sequences of five alpha 1,3-fucosyltransferase and assignment of the chromosomal location of Fuc-TVII gene, together with that acceptor specificity, indicate that Fuc-TVII consists of a unique class of the alpha 1,3-fucosyltransferase family. Determination of the expression levels of these alpha 1,3-fucosyltransferases in various cells revealed that both Fuc-TVII and a myeloid fucosyltransferase Fuc-TIV were significantly expressed in myeloid Lineage cells. Fuc-TVII-transfected Namalwa cells exhibited significant binding to E-selectin in contrast to little binding of the Fuc-TIV-transfected cells. These results suggest that Fuc-TVII may participate in the biosynthesis of the selectin ligands. |
| Document Type: Article |
| Language: English |
Addresses:
1. KYOWA HAKKO KOGYO CO LTD, TOKYO RES LABS, MACHIDA, TOKYO 194 JAPAN |
| Publisher: AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 |
| Subject Category: Biochemistry & Molecular Biology |
| IDS Number: NM065 |
| ISSN: 0021-9258 |
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