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| XIHBOX-8, AN ENDODERM-SPECIFIC XENOPUS HOMEODOMAIN PROTEIN, IS CLOSELY-RELATED TO A MAMMALIAN INSULIN GENE-TRANSCRIPTION FACTOR |
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| Author(s): PESHAVARIA M, GAMER L, HENDERSON E, TEITELMAN G, WRIGHT CVE, STEIN R |
| Source: MOLECULAR ENDOCRINOLOGY Volume: 8 Issue: 6 Pages: 806-816 Published: JUN 1994 |
| Times Cited: 112 References: 44 |
| Abstract: The cis-acting sequences that mediate insulin gene expression exclusively in pancreatic islet beta-cells are localized within the 5'-flanking region between nucleotides -340 and -91. We have identified an evolutionarily conserved, A+T-rich element at -201/-196 basepairs in the rat insulin II gene that is essential for efficient expression in beta-cells. Affinity-purified antibody to the XIHbox 8 protein supershifted the major beta-cell-activator factor complex binding to the -201/-196 element. XIHbox 8 is a Xenopus endoderm-specific homeodomain protein whose expression is restricted to the nucleus of endodermal cells of the duodenum and developing pancreas. Antibody to XIHbox 8 specifically interacts with a 47-kilodalton protein present in this DNA complex. Immunohistochemical studies revealed XIHbox 8-like proteins within the nucleus of almost all mouse islet beta-cells and a subset of islet alpha- and delta-cells. These results are consistent with the proposal that an XIHbox 8-related homeoprotein of 47 kilodalton is required for expression of the mammalian insulin gene in beta-cells. Experiments conducted with antiserum raised to somatostatin transcription factor-1 (STF-1), a recently isolated mammalian XIHbox 8-related homeoprotein, indicate that the STF-1 protein is the mammalian homolog of Xenopus XIHbox 8. |
| Document Type: Article |
| Language: English |
Addresses:
1. VANDERBILT UNIV, MED CTR, SCH MED, DEPT MOLEC PHYSIOL & BIOPHYS, NASHVILLE, TN 37232 USA
2. VANDERBILT UNIV, MED CTR, DEPT CELL BIOL, NASHVILLE, TN 37232 USA
3. SUNY HLTH SCI CTR, DEPT ANAT & CELL BIOL, BROOKLYN, NY 11203 USA |
| Publisher: ENDOCRINE SOC, 4350 EAST WEST HIGHWAY SUITE 500, BETHESDA, MD 20814-4110 |
| Subject Category: Endocrinology & Metabolism |
| IDS Number: NR066 |
| ISSN: 0888-8809 |
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