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| CHROMOSOME-SPECIFIC MICROSATELLITE SETS FOR FLUORESCENCE-BASED, SEMIAUTOMATED GENOME MAPPING |
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| Author(s): REED PW, DAVIES JL, COPEMAN JB, BENNETT ST, PALMER SM, PRITCHARD LE, GOUGH SCL, KAWAGUCHI Y, CORDELL HJ, BALFOUR KM, JENKINS SC, POWELL EE, VIGNAL A, TODD JA |
| Source: NATURE GENETICS Volume: 7 Issue: 3 Pages: 390-395 Published: JUL 1994 |
| Times Cited: 299 References: 19 |
| Abstract: To facilitate large-scale genetic mapping of the human genome, we have developed chromosome-specific sets of microsatellite marker loci suitable for use with a fluorescence-based automated DNA fragment analyser. We present 254 dinucleotide repeat marker loci (80% from the Genethon genetic linkage map) arranged into 39 sets, covering all 22 autosomes and the X chromosome. The average distance between adjacent markers is 13 centiMorgans, and less than 4% of the genome lies more than 20 cM from the nearest marker. Each set of microsatellites consists of up to nine marker loci, with allele size ranges that do not overlap. We selected marker loci on the basis of their reliability in the polymerase chain reaction, polymorphism content, map position and the accuracy with which alleles can be scored automatically by the Genotyper(TM) program. |
| Document Type: Article |
| Language: English |
Addresses:
1. UNIV OXFORD, JOHN RADCLIFFE HOSP, NUFFIELD DEPT SURG, OXFORD OX3 9DU, ENGLAND
2. UNIV OXFORD, NUFFIELD ORTHOPAED CTR, WELLCOME TRUST CTR HUMAN GENET, OXFORD OX3 7LD, ENGLAND
3. GENETHON, F-91000 EVRY, FRANCE |
| Publisher: NATURE PUBLISHING CO, 345 PARK AVE SOUTH, NEW YORK, NY 10010-1707 |
| Subject Category: Genetics & Heredity |
| IDS Number: NU306 |
| ISSN: 1061-4036 |
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